ABSTRACT
ObjectiveUltra-performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry （UPLC-Q-TOF-MS/MS） was used to analyze the chemical constituents in the aerial part and roots of Gentiana straminea from different areas of Qinghai province， and the main chromatographic peaks and differential components of different parts were identified. MethodThe chromatographic separation was performed on a Waters ACQUITY UPLC HSS T3 column （2.1 mm×100 mm， 1.8 μm） with 0.1% formic acid aqueous solution （A）-acetonitrile （B） as the mobile phase for gradient elution （0-1 min， 1%-13%B； 1-5 min， 13%-18%B； 5-7 min， 18%-50%B； 7-9.5 min， 50%-60%B； 9.5-11 min， 60%-99%B； 11-14 min； 99%B； 14-15 min， 99%-1%B； 15-16 min， 1%B）， the column temperature at 40 ℃， and the flow rate of 0.3 mL·min-1. Electrospray ionization （ESI） and negative ion full scan mode were selected for the mass spectrometric conditions to analyze the samples， and the detection range was m/z 50-1 200. Chemical constituents of the aerial part were qualitatively analyzed with the reference substances， literature information and ChemSpider. Principal component analysis （PCA） and orthogonal partial least squares discriminant analysis （OPLS-DA） were used to analyze the classification trend， correlation and differential chemical components between aerial part and roots of G. straminea. ResultA total of 68 components， including 24 iridoids， 13 flavonoids， 8 triterpenoids， 6 xanthones， 5 fatty acids， 4 saccharides， 3 phenolic glycosides， 2 alkaloids， 2 sterols and 1 lignan， were preliminarily identified from the aerial part of G. straminea. Among them， 42 components were firstly reported in 4 Gentiana species included in the 2020 edition of Chinese Pharmacopoeia. Eight differential components were screened out， namely sucrose， maltotriose， loganic acid， shanzhiside methyl ester， 6′-O-β-D-glucosylgentiopicroside， swertiamarin， gentiopicrin and isovitexin. ConclusionThe aerial part of G. straminea is rich in chemical constituents and has good medicinal potential. There were significant differences in the chemical components between the aerial part and roots of G. straminea， and the main differential components were iridoids， which could provide a basis for exploring efficacy differences in different parts of G. straminea.
ABSTRACT
Objective To study the relationship between the distribution of oral ulcer and the syndrome differentiation of Zang-fu viscera. Methods We collected the data of 65 oral ulcer patients admitted in Stomatology Department,the First Affiliated Hospital of Guangzhou University of Chinese Medicine from January of 2015 to July of 2016. The data of emotion,diet,sleep,history of digestive tract disease,and family history of the patients with tongue ulcer and the patients with labio-buccal ulcer were compared. The relationship of the distribution of labio-buccal ulcer,tongue-tip ulcer,and ulcer of lateral part of the tongue with Zang-fu viscera syndrome differentiation was analyzed by statistical software SAS 9.2. Results(1)Tongue ulcer group had higher incidence of anxiety and higher percentage of history of digestive tract disease than labio-buccal ulcer group(P<0.01,P<0.05). (2)Labio-buccal ulcer mostly had a correlation with spleen deficiency blended with dampness or gastrointestinal accumulating heat, the tongue-tip ulcer mostly had a correlation with heart-fire flaring up, and the ulcer of lateral part of tongue mostly had a correlation with pathogenic fire derived from liver-qi stagnation, the difference among the 3 groups being statistically significant(P < 0.01). Conclusion The distribution of oral ulcer has a correlation with Zang-fu viscera syndrome differentiation.